Journal: bioRxiv

Article Title: PROS1 released by human lung basal cells upon SARS-CoV-2 infection facilitates epithelial cell repair and limits inflammation

doi: 10.1101/2024.09.11.612489

Figure Lengend Snippet: A. Confocal 3D scans showing the monocytes on top of the epithelial barrier, at 72 hours cocultures of ALI epithelia with monocytes. B. Lateral view of the 3D scan showing the monocytes on top of epithelial cells, not passing through the tight junctions. C. Dot-plot illustrating that monocytes can be isolated from cocultures with ALI epithelium by expression of CD45 and CD14 D-E. Upon contact with epithelium blood monocytes increased MERTK expression as illustrated MFI of MERTK (D) and by % of MERTK positive cells (E). Each dot represents monocytes from 3 different patients on 3 different ALI epithelial systems (N=3). Statistical analysis was performed using One-Way Anova with Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data is presented as bar plot +/- SD of mean. F. SARS-Cov-2 Infected bronchial cells produced MCSF and CCL2, but no GM- CSF, at 72 hours. Each group is represented by multiple transwell systems that were used as control or infected with SARS-CoV-2, Mock N= 6, SARS-CoV-2 infected epithelia N= 9. Data is presented as bar plot with +/- SD of mean. Statistical comparison was performed using unpaired T test between different conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 G. The IFN-β stimulated epithelium did not affect GM-CSF production. IFN-β pre-treated cocultures had lower levels of GM-CSF compared to controls at 72 hours (left graph). IFN-β stimulation resulted in higher M-CSF production from the epithelium at 24 hours. At 72 hour, the cocultures pre-treated with IFN-β also had higher M-CSF levels than the controls (right). Each group is represented by multiple transwell systems that were used as control or stimulated with 10 ng/mL IFN-β (24 hours stimulated cultures N=4, 72 hours cocultures N= 3). Data is presented as bar plot with mean +/- SD. Statistical comparison was performed using unpaired T test between different conditions. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: Antibodies used include mouse anti-human anti-CD45 (2 μg/mL) (Novus-Bio, NBP2-44863), and rabbit anti-human anti-TJP1 (2.5 μg/mL) (StemCell Technologies, 100-0750).

Techniques: Isolation, Expressing, Infection, Produced, Control, Comparison

Journal: Reproductive Sciences

Article Title: Conservative Hypomethylation of Mesenchymal Stem Cells and Their Secretome Restored the Follicular Development in Cisplatin-Induced Premature Ovarian Failure Mice

doi: 10.1007/s43032-023-01389-4

Figure Lengend Snippet: Phenotype characterization of partially hypomethylated MSCs. The expression of CD105, CD90, CD45, and CD34 was analyzed by flow cytometry for normally methylated cells ( A ), cells treated with 2.5 μM ( B ), or 5 μM ( C ) of 5-Aza-dC. No significant differences were observed in the expression of CD markers between DMSO-treated cells and cells treated with 5-Aza-dC ( D ). Bars represent the average (±SD) of three independent measurements, ns , not significant. Comparison between means was performed by one-way ANOVA test

Article Snippet: Phycoerthrin (PE)-conjugated mouse monoclonal antibodies of CD90, and CD34 or FITC-conjugated monoclonal antibodies of CD105 and CD45 were from (Biotechne R&D System, MN, USA).

Techniques: Expressing, Flow Cytometry, Methylation, Comparison

Journal: Scientific Reports

Article Title: Genome-based reclassification of Micromonospora veneta Kaewkla et al. 2022 as a later heterotypic synonym of Micromonospora coerulea Jensen 1932 (Approved lists 1980)

doi: 10.1038/s41598-025-13676-y

Figure Lengend Snippet: The length of the 16S rRNA gene sequences of M. coerulea JCM 3175 T and M. veneta DSM 109713 T are 1,436 bp and 1,438 bp respectively. Maximum-likelihood tree based on 16S rRNA gene sequences, showing the phylogenetic positions of strains DSM 109713 T and JCM 3175 T and related members within the genus Micromonospora . Actinoplanes aksuensis TRM 88003 T (OM 112204) was used as an outgroup. Bootstrap values (expressed as percentages of 1000 replicates) above 50% are shown at the branch points. Bar, 0.01 substitutions per nucleotide position.

Article Snippet: At the time of writing, based on Parte the genus Micromonospora includes 130 species with validly published names ( https://lpsn.dsmz.de/genus/micromonospora ), widely distributed in various environments, including peat swamp forests root noduless hot spring soils and deep sea environments .

Techniques:

Journal: Scientific Reports

Article Title: Genome-based reclassification of Micromonospora veneta Kaewkla et al. 2022 as a later heterotypic synonym of Micromonospora coerulea Jensen 1932 (Approved lists 1980)

doi: 10.1038/s41598-025-13676-y

Figure Lengend Snippet: Pangenome analysis of the 16 Micromonospora type strains. (A) A pangenome map depicting the functional distribution of core gene clusters and unique genes in the selected Micromonospora genomes. (B) The accumulative curve showing the number of core gene clusters in relation to the number of genomes included in the pangenome analysis. The blue line represents the change in number of core gene clusters as the number of genomes included in the pan-genome analysis increases. The orange line typically indicates the number of non-core gene clusters (or gene clusters, variable gene clusters) as the number of genomes included changes. (C) UpSet plot illustrating the unique genes as well as the genes shared between the Micromonospora strains.

Article Snippet: At the time of writing, based on Parte the genus Micromonospora includes 130 species with validly published names ( https://lpsn.dsmz.de/genus/micromonospora ), widely distributed in various environments, including peat swamp forests root noduless hot spring soils and deep sea environments .

Techniques: Functional Assay